scRNA-seq

UMAP/Clustering


Patient data

  • Filtering

    • UMI >= 1000
    • Mitochondrial content <= 10
    • Doublet removal : Yes (scrublet)

UMAP

UMAP by treatment

Split by sample

Distribution of number of cells

Clustering

  • resolution 0.2
  • resolution 0.4

Resolution 0.2 UMAP

Resolution 0.2 UMAP (label)

Cell numbers

Proportion by sample

Proportion by cluster

Resolution 0.4 UMAP

Resolution 0.4 UMAP (label)

Cell numbers

Proportion by sample

Proportion by cluster

Sankey plot

Markers

  • resolution 0.2
  • resolution 0.4

Markers (0.2)

Download cluster marker file
(no download on example version)

Heatmap (res 0.2)

Top 5 genes from each cluster

Markers (0.4)

Download cluster marker file
(no download on example version)

Heatmap (res 0.4)

Top 5 genes from each cluster